Biofilm Detection of Staphylococcus Aureus Isolated from Oral Infections

Abstract

This work involves identifying biofilm development by Staphylococcus aureus isolated from oral infections. Biofilm formation transpires due to bacterial activity influenced by multiple elements that create an oral environment conducive to its development, as biofilm formation is a critical pathogenicity factor for bacteria. This investigation involved the collection of forty clinical samples from the oral cavities of individuals with oral issues. Staphylococcus aureus was isolated and identified using several methods, with confirmation via polymerase chain reaction (PCR), yielding 28 positive samples and 12 negative samples. Bacteria can establish biofilms on biological and abiotic surfaces in both natural and clinical settings. Bacterial aggregation within biofilms is created by the extracellular matrix they secrete. Staphylococcus aureus is a common pathogen linked to biofilm infection. The assessment of biofilm formation on polymeric surfaces by Staphylococcus aureus isolates was conducted utilizing the crystal violet microtiter plate technique. Crystal violet is a negative stain that exhibits affinity for positively charged molecules on the cell surface, nucleic acids, and polysaccharides. The extent of biofilm formation was quantified using optical density (OD) values, which were compared to the control OD (ODc); the classifications resulted in 21 highly adherent samples, 8 lightly adherent, 49 barely adherent and 12 nonadherent.